Abcam, ab279415, Human Ribonuclease 3 ELISA Kit (ECP)

Size: 1 x 96Tests
Human Ribonuclease 3 ELISA Kit (ECP) is a single-wash 90-min Simplestep used to quantify Human Ribonuclease 3 (ECP) with a sensitivity of 1.265 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.265 pg/mL,
Range:9.375 - 600 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Ribonuclease 3 ELISA Kit (ECP) ab279415 is a rapid single-wash 90-min Sandwich ELISA to measure Human Ribonuclease 3 (ECP)in cell culture supernatant, cell lysate, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.265 pg/mL.
How the assay works
Human Ribonuclease 3 (ECP)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Ribonuclease 3 ELISA Kit (ECP) ab279415 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Ribonuclease 3, also known as Eosinophil Cationic Protein (ECP) is part of the Ribonuclease A family and is able to degrade RNA. Ribonuclease 3 is expressed by the eosinophils and secreted into the blood stream. Serum and plasma levels of Ribonuclease 3 increase in response to inflammatory diseases. Ribonuclease 3 is measured diagnostically to diagnose allergic disorders and other conditions connected with activated eosinophils.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ribonuclease 3 commonly known as Eosinophil Cationic Protein (ECP) is an enzyme with ribonuclease activity. Researchers sometimes refer to it as human RNase 3. The protein has a molecular mass of about 18 to 22 kDa and is highly expressed in eosinophils a type of white blood cell primarily involved in allergic responses and parasitic infections. ECP exhibits cytotoxic bactericidal and antiviral activities contributing to its diverse biological functions.
Biological function summary
Ribonuclease 3 plays a significant role in immune defense mechanisms leveraging its arginine-rich domain to interact with negatively charged microbial surfaces. This interaction allows ECP to participate in the destruction of pathogen cell walls. It is not part of a larger complex but interacts with cellular membranes making it an effective tool against a variety of pathogens. Whether acting through enzymatic activity or by disrupting membrane integrity ECP maintains a pivotal role in host defense.
Pathways
ECP influences processes in both innate and adaptive immune responses. It actively participates in the Th2 cell-mediated immune response which is critical for countering parasitic infections. This protein connects with other components like major basic protein and eosinophil peroxidase through these pathways forming an integrated network that boosts immune responses. Its presence in pathways linked to inflammation and immunity highlights its multifunctionality and importance.
ECP levels correlate with conditions such as asthma and eosinophilic esophagitis. In asthma elevated ECP levels indicate eosinophilic inflammation and airway hyperresponsiveness. Researchers have identified a connection between ECP and proteins like interleukin-5 which modulate eosinophil activity. Similarly in eosinophilic esophagitis increased ECP levels indicate ongoing inflammation and tissue damage providing insights into disease progression and severity.