Abcam, ab279732, Phospho-AKT / GSK3 beta / mTor ELISA kit

Size: 1 x 96Tests
Phospho-AKT / GSK3 beta / mTor ELISA kit is a Semi-quantitative ELISA for the measurement of Phospho-AKT / GSK3 beta / mTor in Human, Mouse in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Mouse, Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-AKT / GSK3 beta / mTor ELISA Kit (ab279732) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated AKT/ GSK3 beta / mTor protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. Samples are pipetted into the wells and target protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and a detection antibody is used to detect the captured target protein. After washing away unbound antibody, an HRP-conjugated secondary antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of target protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MTOR also known as mechanistic target of rapamycin FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1) or mammalian target of rapamycin is a serine/threonine protein kinase with a mass of approximately 289 kDa. It plays an important role in cell growth proliferation motility and survival. mTOR is part of two distinct complexes mTORC1 and mTORC2 which are different in composition and function. mTOR is expressed in various tissues including the brain liver muscle and adipose tissue.
Biological function summary
MTOR is a central component of cell signaling pathways that respond to nutrient and energy status. It is part of the mTOR complexes mTORC1 and mTORC2 with each complex having distinct regulatory inputs and outputs. AKT1 also known as Protein Kinase B (PKB) acts upstream of mTOR activating mTORC1 which regulates protein synthesis through downstream effectors like S6 kinase. GSK3 beta (Glycogen Synthase Kinase 3 beta) with a mass of roughly 46 kDa is known to interact inversely with AKT1. Phospho-AKT the phosphorylated form of AKT1 plays a role in the activation of mTOR and the inhibition of GSK3 beta.
Pathways
MTOR AKT1 and GSK3 beta are key players in the PI3K/AKT/mTOR signaling pathway which is important for regulating cell growth and survival. The pathway involves PI3K activating AKT1 which in turn activates mTORC1 facilitating cellular anabolic processes. AKT1 phosphorylation inhibits GSK3 beta decreasing its activity in glycogen synthesis regulation. Additionally mTOR is involved in the insulin signaling pathway where it helps coordinate cell responses to insulin influencing glucose homeostasis.
Dysregulation of mTOR GSK3 beta and AKT1 has been associated with cancer and diabetes. Overactive mTOR signaling links to many cancers due to its role in promoting cell proliferation and survival. GSK3 beta when highly active contributes to insulin resistance and type 2 diabetes often acting in concert with aberrant mTOR and AKT1 signaling. Therapeutic targeting of these components within their pathways shows promise in treating these conditions with interventions often aiming to restore balance among these interacting proteins.