Abcam, ab279879, Phospho-S6K1 (T421/S424) ELISA Kit

Size: 1 x 96Tests
Phospho-S6K1 (T421/S424) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-S6K1 (T421/S424) in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-S6K1 (T421/S424) ELISA Kit (ab279879) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated S6K1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-S6K1. An anti-pan S6K1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and S6K1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-S6K1 (T421/S424) antibody is used to detect phosphorylated S6K1. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of S6K1 (T421/S424) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S6K1 also known as p70S6K is a serine/threonine kinase involved in protein synthesis. The protein has a molecular weight of approximately 70 kDa. Scientists often examine the presence of S6K1 in various tissues as it is expressed ubiquitously in eukaryotic cells. This kinase plays an instrumental role in the regulation of cell growth proliferation and survival by phosphorylating ribosomal protein S6 and other targets. Researchers explore various cellular contexts where S6K1 activity is implicated due to its widespread expression and function.
Biological function summary
S6K1 functions as a downstream effector of the mammalian target of rapamycin (mTOR) complex specifically mTORC1. S6K1 interacts with several protein complexes enhancing its regulatory capacity in cellular activities. It modulates protein synthesis by phosphorylating substrates involved in translation. By controlling these processes S6K1 aids in cell size regulation and energy metabolism which are critical for maintaining cellular homeostasis and adaptation to nutrient availability.
Pathways
S6K1 plays an essential role in the mTOR signaling pathway a central regulator of cell growth and metabolism. S6K1 functions in close connection with proteins such as mTOR and Raptor within this pathway. Additionally it is involved in the insulin signaling pathway where it works with proteins like insulin receptor substrate (IRS). Both pathways highlight S6K1's role in nutrient sensing and response emphasizing its significance in energy and protein homeostasis.
S6K1 is associated with conditions such as cancer and type 2 diabetes. Dysregulation of S6K1 activity can lead to abnormal cell proliferation contributing to oncogenesis in various cancer types. Specifically altered signaling through pathways involving mTOR and Akt proteins connects S6K1 to cancer progression. In type 2 diabetes S6K1 affects insulin sensitivity where overactive S6K1 signaling leads to insulin resistance. Here interactions with insulin receptor substrate proteins highlight its influence on metabolic disorders underlining its critical role in disease pathology.