Abcam, ab279954, Phosphotyrosine Tec ELISA Kit

Size: 1 x 96Tests
Phosphotyrosine Tec ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine Tec in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phosphotyrosine Tec ELISA Kit (ab279954) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Tec protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-Tec. An anti-Tec antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Tec present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiphosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Tec bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tec also known as Tec protein tyrosine kinase functions as a non-receptor tyrosine kinase involved in signal transduction. Its molecular mass is approximately 72 kDa. Tec is expressed predominantly in hematopoietic cells such as B cells T cells and myeloid lineage cells. This protein plays a significant role in mediating cellular communication by transferring phosphate groups from ATP to specific tyrosine residues on target proteins.
Biological function summary
Tec influences cellular processes like proliferation differentiation and survival. Tec belongs to the Tec kinase family which also includes Btk Itk Rlk and Bmx. Though Tec does not form permanent complexes it interacts dynamically with various signaling proteins. These interactions allow Tec to modulate immune cell development and function significantly impacting the adaptive immune response.
Pathways
Tec is an integral part of the B-cell receptor (BCR) signaling pathway and the PI3K/Akt signaling pathway. Upon activation of BCR Tec aids in signal propagation leading to B cell activation and proliferation. Tec operates synergistically with proteins like Src family kinases leading to the activation of downstream signaling pathways. The protein also facilitates the Akt pathway which controls cell growth and survival by interacting with phosphatidylinositol 3-kinase (PI3K).
Tec links to immunodeficiencies and cancers such as leukemia. Dysfunctional Tec signaling can impair immune responses contributing to conditions like X-linked agammaglobulinemia a disorder often connected to mutations in Bruton's tyrosine kinase (Btk). Overactive Tec signaling may promote leukemogenesis linking Tec to leukemias where it cooperates with proteins such as STAT5 which is involved in cell growth and survival pathways.