Abcam, ab280090, Anti-PDPK1 antibody [5/PDK1] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal PDPK1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5/PDK1,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab280090 is the carrier-free version of
ab280085
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDPK1 also known as 3-phosphoinositide-dependent protein kinase-1 or PDK1 is a protein kinase with a molecular mass of approximately 63 kDa. It plays an important mechanical role in the activation of other kinases through phosphorylation on threonine or serine residues. PDPK1 is widely expressed in various tissues including heart lung pancreas and liver reflecting its significant roles in cellular signalling.
Biological function summary
PDPK1 is a master regulator of several AGC kinase family enzymes. It functions as a docking station coordinating with other proteins for activation processes. For example PDPK1 helps in activating PKB/Akt which is required for cell survival and growth. PDPK1 forms part of signaling complexes that manage processes like metabolism growth and survival.
Pathways
PDPK1 plays an essential role in the PI3K/AKT pathway which is an important signaling pathway for cellular growth and survival. This pathway begins with the activation of PI3K leading to the production of PIP3 which creates a binding site for PDPK1 at the plasma membrane where it activates PKB/Akt. Also PDPK1 interacts with NF-κB pathway components linking it to processes in immune response regulation and inflammation.
PDPK1 has associations with cancer and Type 2 diabetes. Abnormal activity of PDPK1 is observed in several cancer types indicating its role in uncontrolled cell growth. In Type 2 diabetes PDPK1 influences insulin signaling disruptions and glucose metabolism. PDPK1 connects these conditions through interactions with proteins like mTOR which influences cell growth and metabolism further exemplifying its importance in disease mechanisms.