Abcam, ab280575, Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SIK2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22722-23,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab280575 is the carrier-free version of
ab245211
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIK2 also known as Salt-Inducible Kinase 2 is a protein kinase involved in various cellular processes. It has a molecular mass of approximately 94 kDa. SIK2 is expressed in many tissues with high levels found in adipose tissue liver and skeletal muscle. As a kinase it phosphorylates specific target proteins regulating their activity and influencing cellular mechanisms.
Biological function summary
SIK2 participates in energy metabolism and cellular response to external stress. It interacts with several co-factors to form active complexes facilitating its activity. This protein regulates lipid and carbohydrate metabolism therefore playing a significant role in maintaining cellular energy homeostasis. Additionally SIK2 has a function in controlling the inflammatory response providing a link between metabolism and immune function.
Pathways
SIK2 acts as an essential mediator in the AMPK signaling pathway. This pathway is important for energy balance within cells and is related to other kinases like AMPK and other SIK family members. SIK2 also contributes to the regulation of the cAMP signaling pathway which is linked to various physiological processes. Through these pathways SIK2 influences processes such as glucose uptake and fat oxidation and interacts with proteins like CREB and HDACs.
SIK2 has implications in conditions like obesity and type 2 diabetes. Dysregulation of SIK2 activity can lead to metabolic imbalance and insulin resistance. This kinase is also associated with cancer progression particularly in ovarian cancer where it influences cell proliferation. In these diseases SIK2's connection to proteins such as AMPK and CREB suggests a complex role in disease modulation and potential therapeutic targeting.