Abcam, ab281018, Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR22815-150] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Glutathione Peroxidase 3/GPx-3 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22815-150,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab281018 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse GPX3. The recommended pair for sandwich ELISA is:
Capture:
ab281168
, Mouse GPX3 Capture Antibody (unconjugated)
Detector: ab281018, Mouse GPX3 Detector Antibody (unconjugated)
The reference range value is 0.39-50 ng/mL.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione Peroxidase 3 also known as GPx-3 or 3GPx is an antioxidant enzyme important for protecting cells from oxidative damage by reducing hydrogen peroxide and organic hydroperoxides. It is a member of the glutathione peroxidase family and has a molecular mass of approximately 23 kDa. GPx-3 is expressed mainly in the kidney and subsequently released into the bloodstream playing an important role in its extracellular antioxidant defense mechanism.
Biological function summary
GPx-3 helps maintain cellular redox balance and is involved in detoxifying harmful oxidative agents. It does not form part of any larger complex but works independently to reduce peroxides with the help of glutathione an important reducing agent. GPx-3 has significant activities in plasma and extracellular fluids indicating its role in protecting the body from oxidative stress away from cellular environments.
Pathways
GPx-3 functions in the glutathione metabolic pathway and the defense system against oxidative stress. It works together with proteins such as glutathione reductase to recycle oxidized glutathione back to its reduced form allowing continuous protection against reactive oxygen species. This pathway is essential for maintaining balance between oxidative stress and antioxidant defense in the organism.
GPx-3 has been linked to cardiovascular diseases and certain types of cancer. Low levels of GPx-3 in plasma have been associated with increased risk of thrombosis as antioxidant defenses are compromised. In cancer studies altered GPx-3 expression is often observed thought to affect the progression of disease by changing the cellular redox environment. Other proteins that interact with GPx-3 during disease conditions include thioredoxin and catalase both influencing and being regulated by redox states in diseased tissues.