Abcam, ab281054, Anti-Furin antibody [EPR23363-155] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FURIN antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23363-155,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab281054 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Furin. The recommended pair for sandwich ELISA is:
Capture:
ab281204
, Human Furin Capture Antibody (unconjugated)
Detector: ab281054, Human Furin Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Furin is a serine endoprotease that plays critical role in the cleavage of precursor proteins into their active forms. It belongs to the proprotein convertase family and is also known as furin protease. The molecular weight of furin is approximately 54 kDa. Furin is ubiquitously expressed in various tissues in the body with high expression levels in the liver and the central nervous system. Furin is mainly located in the trans-Golgi network where it processes many precursor proteins involved in various cellular functions.
Biological function summary
Furin processes precursor proteins by cleaving at specific sequence motifs. This activity is essential for the maturation of many hormones growth factors and receptors. Furin is not part of a larger complex; it functions independently in its proteolytic activity. The processing by furin ensures that proteins become functional affecting numerous biological processes across different cell types and tissues.
Pathways
The activity of furin impacts the regulation of the Notch signaling pathway and the MAPK/ERK pathway. In the Notch signaling pathway furin processes Notch receptors facilitating their activation and downstream signaling. In the MAPK/ERK pathway furin activates several key proteins including growth factors and receptors essential for cell proliferation and differentiation. Furin-related proteins such as convertase enzymes are part of a similar family having roles in overlapping or complementary pathways.
Furin is implicated in several conditions including cardiovascular diseases and certain cancers. In cardiovascular diseases furin's role in processing proteins such as pro-BNP may have implications in cardiac function and disease progression. In cancer altered furin activity can lead to the inappropriate activation of growth factors and receptors contributing to tumor progression. Furin inhibitors are researched for their potential to modulate furin activity in these diseases and provide therapeutic benefits.