Abcam, ab28146, Anti-Cytochrome P450 2E1 antibody

Size: 100µL
Anti-Cytochrome P450 2E1 antibody (ab28146) is a rabbit polyclonal antibody detecting Cytochrome P450 2E1 in Western Blot . Suitable for Human, Mouse, Rabbit, Rat . - Over 150 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Rabbit, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Rat Cyp2e1.P05182,
Specificity:The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.

Product details:
What is this antibody validated in?
Anti-Cytochrome P450 2E1 antibody (ab28146) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rabbit, Rat samples.
What is the molecular weight of Cytochrome P450 2E1?
Anti-Cytochrome P450 2E1 (ab28146) specifically detects a band for Cytochrome P450 2E1 (UniProt: P05181) at a molecular weight of 50-55kDa.
Trusted by the scientific community
Anti-Cytochrome P450 2E1 (ab28146) was first used in a scientific publication in 2006 and has been cited over 150 times in peer-reviewed journals.
Reviewed by scientists
Anti-Cytochrome P450 2E1 (ab28146) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome P450 2E1 also known as CYP2E1 is an enzyme belonging to the cytochrome P450 superfamily. It weighs approximately 56 kDa. This enzyme is majorly expressed in the liver but can also be found in other tissues like the kidney and lungs. It plays a central role in the oxidation and metabolism of various endogenous and exogenous substances including fatty acids drugs and alcohols.
Biological function summary
The enzyme CYP2E1 metabolizes low-molecular-weight compounds. It catalyzes the conversion of substrates to more soluble forms enabling their excretion from the body. It is not a part of a larger protein complex but often partners with NADPH-cytochrome P450 reductase in electron transfer. CYP2E1 can also produce reactive oxygen species during its catalytic cycle which may impact cellular structures and processes.
Pathways
CYP2E1 is involved in the oxidative metabolism of ethanol and other xenobiotics. It participates in the metabolic pathways for drugs and carcinogens transforming them into either active or inactive metabolites. The enzyme has connections with other P450 enzymes such as CYP3A4 within the same metabolic landscape often overlapping substrates and regulatory mechanisms.
CYP2E1 plays a significant role in alcohol-induced liver injury because its metabolism of ethanol generates toxic by-products like acetaldehyde and reactive oxygen species contributing to liver damage. The enzyme is also associated with the development of certain cancers as its metabolism of procarcinogens can produce carcinogenic metabolites. Other related cytochrome P450 enzymes such as CYP1A2 share pathways involved in carcinogen activation highlighting an interconnected network contributing to disease progression.