Abcam, ab282030, Anti-ARL8A + ARL8B antibody [EPR24376-103] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ARL8A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24376-103,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab282030 is the carrier-free version of
ab281997
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARL8A and ARL8B also known as ADP-ribosylation factor-like protein 8A and 8B are small GTPases with masses of approximately 21 kDa. They belong to the ARF-like GTPase family and they localize mainly on lysosomes and late endosomes. Both ARL8A and ARL8B widely express in tissues but show higher levels in certain types like immune cells. Their structural and functional similarities allow them to share roles in cellular functions related to lysosomal positioning and movement.
Biological function summary
ARL8A and ARL8B regulate the anterograde transport of lysosomes and are part of the lysosomal trafficking complex. They help in organizing lysosomal positioning a role important for normal lysosome function. This function includes interaction and formation of a complex with other proteins such as PLEKHM2 and SKIP which facilitate the attachment of lysosomes to microtubule tracks via kinesin motor proteins. This movement allows lysosomes to fulfill their roles in cellular distribution and degradation processes.
Pathways
ARL8A and ARL8B play roles in the lysosomal protein transport pathway and autophagy. They engage in lysosomal motility impacting autophagic flux a process essential for cellular homeostasis and stress response. ARL8B in particular interacts with the HOPS complex promoting fusion between autophagosomes and lysosomes therefore influencing autophagy. Among related proteins Rab7A is noteworthy as a regulator of late endosomal trafficking pathways that interface with processes involving ARL8 proteins.
ARL8A and ARL8B are associated with neurodegenerative diseases and cancer. Dysregulation in lysosomal positioning can impair autophagy linking these proteins to neurodegenerative conditions like Parkinson's disease. In cancer altered lysosomal trafficking influences tumor cell invasion and metastasis. Both proteins interact with LAMP proteins which contribute to the lysosomal membrane integrity central to diseases involving lysosomal dysfunction.