Abcam, ab282161, Alexa Fluor® 488 Anti-SARS CoV-2 ORF3a Protein antibody [EPR24848-9]

Size: 100µL
Rabbit Recombinant Monoclonal SARS CoV-2 ORF3a Protein antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell line - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24848-9,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 ORF3a protein also known as ORF3 is an accessory protein of the SARS-CoV-2 virus weighing approximately 275 amino acids. It is expressed in infected host cells and plays an important role in the virus's lifecycle. ORF3a is known for its role as an ion channel involved in the viral replication cycle. Its functions include altering host cell processes to facilitate viral propagation and possibly contributing to viral pathogenesis.
Biological function summary
The SARS-CoV-2 ORF3a protein interferes with the host immune response promoting virus survival and replication. ORF3a is implicated in the enhancement of viral pathogenic effects by modulating innate and adaptive immunity. Though not part of a large known stable complex ORF3a interacts with other viral and host proteins impacting processes like apoptosis and inflammation.
Pathways
ORF3a affects several critical processes within the host cell. It plays a role in the apoptosis pathway influencing cell death mechanisms that help the virus evade immune defenses. Additionally it modulates the NF-kB signaling pathway which is vital for immune response regulation. Other related proteins include ORF7a and NSP6 which work alongside ORF3a to manipulate host cell environments to the virus's advantage.
The SARS-CoV-2 ORF3a protein is directly connected to COVID-19 impacting the severity and progression of the disease. Its interaction with host proteins contributes to dysregulated immune responses and cytokine storms characteristic of severe COVID-19 cases. The protein's role in apoptosis links it to potential lung tissue damage exacerbating respiratory conditions associated with COVID-19 infections.