Abcam, ab282297, Mouse Prealbumin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Mouse Prealbumin ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Mouse Prealbumin (Transthyretin, TTR) with a sensitivity of 62.48 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader - Different formats for different needs: 384-well for higher throughput - Need to design your own assay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 62.48 pg/mL,
Range:234.38 - 15000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Prealbumin ELISA Kit ab282297 is a rapid single-wash 90-min ELISA kit to measure Mouse Prealbumin in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.
How the assay works
Mouse Prealbumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of mouse Prealbumin. The standard protein in this kit is full length mouse Prealbumin. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and tissue culture supernatant sample types. Cell extract, tissue extract, urine, saliva, CSF, and milk samples have not been tested with this kit.
Mouse Prealbumin ELISA Kit ab282297 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Save your precious samples
Our 384-well Mouse Prealbumin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Prealbumin Antibody Pair - BSA and Azide free
ab253737
- Anti-Prealbumin antibody [EPR20971-130] - BSA and Azide free (Capture)
ab283848
- Anti-Prealbumin antibody [EPR20971-140] - BSA and Azide free (Detector)
ab283849
Related and recommended products
Mouse Prealbumin ELISA Kit ab282297 is commonly used to study nutrition and endocrine disorders.
To measure prealbumin in human samples:
- Human Prealbumin ELISA kit
ab231920
Prealbumin (Transthyretin, or TTR) is a transport protein for thyroxine and retinol-binding protein bound to retinol. Prealbumin forms a dimer of dimers, and misfolding of it is associated with various amyloid diseases. Prealbumin levels are an indication of protein-calorie nutrition and has been used to monitor the effectiveness of parenteral nutrition.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C