Abcam, ab282307, Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5)

Size: 1 x 96Tests
Human Tartrate Resistant Acid Phosphatase ELISA Kit (ACP5) is a single-wash 90-min Simplestep used to quantify Human Tartrate Resistant Acid Phosphatase (ACP5) with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8 pg/mL,
Range:93.75 - 6000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Tartrate Resistant Acid Phosphatase ELISA kit (ab282307) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Tartrate Resistant Acid Phosphatase protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tartrate Resistant Acid Phosphatase with 8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tartrate Resistant Acid Phosphatase, also known as Type 5 Acid Phosphatase (ACP5) is a lysosomal phosphatase involved in osteopontin/bone sialoprotein dephosphorylation. The expression of Type 5 Acid Phosphatase seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tartrate Resistant Acid Phosphatase (TRAP) also known as ACP-5 and type 5 acid phosphatase is an enzyme with mass approximately 35 kDa. It is expressed in osteoclasts macrophages and dendritic cells playing a role in bone resorption and macrophage activation. TRAP exists in both a monomeric and proteolytically activated dimeric form. Its unique tartrate resistance distinguishes it from other acid phosphatases providing specificity for enzyme assays and studies.
Biological function summary
TRAP contributes significantly to bone remodeling through its action in osteoclasts. It helps in degrading mineralized matrix components and may also serve a role in dendritic cell activation signaling and macrophage-related processes. TRAP forms part of a complex mechanism that includes catabolic enzymes which work together to ensure normal skeletal function and immune response.
Pathways
TRAP plays an integral role in the bone resorption pathway along with proteins such as cathepsin K and matrix metalloproteinases. It is involved in osteoclast differentiation necessary for bone turnover and homeostasis. Additionally it participates in the immune response through its activity in macrophages affecting inflammation pathways and innate immune functions.
Abnormal TRAP activity associates with osteopetrosis and osteoporosis. These diseases affect bone density and strength with TRAP often showing elevated levels in conditions of high bone turnover. TRAP activity also connects to inflammatory disorders due to its expression in macrophages linking it to proteins like RANKL and osteoprotegerin which influence bone metabolism and immune signaling.