Abcam, ab282864, Human SORL1 ELISA Kit

Size: 1 x 96Tests
Human SORL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SORL1 with a sensitivity of 8.56 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.56 pg/mL,
Range:62.5 - 4000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human SORL1 ELISA Kit ab282864 is a rapid single-wash 90-min Sandwich ELISA to measure Human SORL1 in cell culture supernatant, cell lysate, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 8.56 pg/mL.
How the assay works
Human SORL1 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human SORL1 ELISA Kit ab282864 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-SORL1 antibody [EPR23262-73] - BSA and Azide free (Capture)
ab273560
- Anti-SORL1 antibody [EPR23262-52] - BSA and Azide free (Detector)
ab273560
Human neuronal sortilin-related receptor (SORL1) is a sorting receptor that directs several proteins to their correct location within the cell for sorting and processing. SORL1 is linked to regulation of intracellular trafficking and processing of amyloid precursor protein by impairing the cleavage of APP through a-secretase, b-secretase, and c-secretase in leading to reduced levels of soluble APP and amyloid beta protein,the major component of amyloid plaques. SORL1 has been linked to protective effects against amyloidogenesis in Alzheimer's disease. Human SORL1 share 93.7%, 93.5%, 92.5%, and 98.4% sequence homology with mouse, rat, bovine, and monkey, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C