Abcam, ab282866, Human KAT3B / p300 ELISA Kit

Size: 1 x 96Tests
Human KAT3B / p300 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human KAT3B / p300 with a sensitivity of 51 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 51 pg/mL,
Range:94 - 6000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human KAT3B ELISA kit (ab282866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human KAT3B protein in human cell culture samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human KAT3B with 51 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
.html") is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Histone acetyltransferase p300 (KAT3B/P300), also known as p300 HAT and KAT3B, is an enzyme encoded by the KAT3B/P300 gene. KAT3B/P300 functions as histone acetyltransferase that regulates transcription via chromatin remodeling. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. KAT3B/P300 has also been shown to be a co-activator of HIF1A (hypoxia-inducible factor 1 alpha) and plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene have been thought to be associated to Rubinstein-Taybi syndrome, Menke-Hennekam syndrome 2, and epithelial cancer.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KAT3B also known as p300 is a histone acetyltransferase with a molecular weight of approximately 300 kDa. Known for its ability to acetylate histone and non-histone proteins p300 modulates gene expression by altering chromatin structure. The protein is ubiquitously expressed across various tissues impacting numerous cellular processes by its mechanical functions of modifying chromatin.
Biological function summary
KAT3B/p300 influences multiple cellular activities like cell growth differentiation and apoptosis. It functions as a transcriptional coactivator and integrates various signaling pathways into the cell's gene expression program. p300 often forms complexes with other transcription factors enhancing or repressing their activity based on the cellular context. By modifying transcription factors it plays an essential role in controlling cell fate decisions.
Pathways
P300 participates in the regulation of significant cellular processes including the Wnt signaling pathway and the p53 pathway. In the Wnt signaling pathway p300 acts alongside beta-catenin to regulate gene expression while in the p53 pathway it acetylates the p53 protein influencing the cell’s response to DNA damage. These pathways highlight p300's interaction with important proteins such as CBP (CREB-binding protein) which shares a similar functional repertoire and works synergistically in gene regulation.
P300 is implicated in cancers and congenital disorders. In cancer aberrant regulation or mutation of p300 can lead to uncontrolled cell proliferation and tumor growth; p300 inhibitors like C646 are researched for their therapeutic potential. Additionally Rubinstein-Taybi syndrome a congenital disorder results from mutations in the p300 gene showcasing p300’s importance in developmental processes. The interplay of p300 with proteins such as CBP is also relevant in these conditions where disrupted interactions may lead to disease manifestation.