Abcam, ab282877, Human Retinoic Acid Receptor alpha ELISA Kit (RARA)

Size: 1 x 96Tests
Human Retinoic Acid Receptor alpha ELISA Kit (RARA) is a single-wash 90-min Simplestep used to quantify Human Retinoic Acid Receptor alpha (RARA) with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 10 pg/mL,
Range:54.69 - 3500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Retinoic Acid Receptor alpha ELISA kit (ab282877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Retinoic Acid Receptor alpha protein in human cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Retinoic Acid Receptor alpha with 10 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Retinoic Acid Receptor alpha, also known as RARA and NR1B1, is nuclear receptor and transcription factor which plays a key role in cell growth and differentiation. With its binding partner RXR, RARA binds retinoic acid and removes NCOR1 and SMRT transcriptional repression. The immunogen from which the antibodies in this kit were raised represent the C-terminal half of the mature protein. This protein is homologous with many species, so cross-reactivity is expected.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Retinoic Acid Receptor alpha (RARA) a member of the nuclear receptor superfamily functions as a transcription factor activated by retinoic acid. Commonly referenced in literature RARA has an approximate molecular mass of 50 kDa. It is expressed in a variety of tissues including the liver the lung and the immune system. By forming heterodimers with retinoid X receptors RARA regulates the transcription of genes linked to cell differentiation proliferation and apoptosis.
Biological function summary
RARA plays an essential role in mediating the effects of retinoic acid in the body. It is part of a larger receptor complex that interacts with co-regulators to modulate gene expression. This process is significant for embryonic development and the maintenance of normal physiological functions. Through its action RARA contributes to the proper development of organs and is critical for maintaining immune homeostasis and enabling the cellular response to environmental changes.
Pathways
RARA's activity impacts important signaling routes such as the retinoic acid signaling pathway and the Wnt signaling pathway. It collaborates with proteins like retinoid X receptors (RXRs) and other nuclear receptors to influence gene expression processes. These pathways maintain cellular differentiation and tissue homeostasis demonstrating RARA's integrative role in cellular signaling and communication.
RARA's dysregulation has been linked to acute promyelocytic leukemia (APL) and some autoimmune diseases. In APL aberrant fusion proteins involving RARA disrupt normal transcriptional regulation leading to malignant transformation. Additionally the interaction of RARA with proteins such as promyelocytic leukemia protein (PML) further influences the disease's development. Research on these associations highlights the therapeutic potential of targeting RARA activities for disease intervention and treatment strategies.