Abcam, ab282882, Mouse ALT ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Mouse ALT ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify mouse alanine aminotransferase (ALT) with a sensitivity of 4 pg/mL. The assay follows a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric Sandwich ELISA – 450 nm readout, compatible with any standard plate reader - Different formats for different needs – 384-well format available for high-throughput applications - Broad sample compatibility – works with serum, plasma - Cited in over 15 publications
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 4 pg/mL,
Range:46.88 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse ALT ELISA Kit ab282882 is a rapid single-wash 90-min ELISA kit to measure Mouse ALT in serum and plasma. This SimpleStep ELISA® sensitivity is 4 pg/mL.
How the assay works
Mouse ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of mouse ALT. The standard protein in this kit is full length mouse ALT. Native signal was detected in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)sample types. Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.
Mouse ALT ELISA Kit ab282882 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse ALT ELISA Kit ab282882
Mouse ALT ELISA Kit
ab28288
has been used to study mitochondrial homeostatis in liver disease
and the effects of iron deficiency in cellular response to malaria
References:
Ai-Lei Xu et al. 2024, PMID: 37902450,
Hal Drakesmith et al. 2023, PMID: 37812650
Save your precious samples
Our 384-well Mouse ALT ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Alanine aminotransferase (ALT) Antibody Pair - BSA and Azide free
ab253647
- Anti-Alanine Transaminase antibody [EPR21013-71] - BSA and Azide free (Capture)
ab281207
- Anti-Alanine Transaminase antibody [EPR21013-202] - BSA and Azide free (Detector)
ab281057
Related and recommended products
Mouse ALT ELISA kit
ab234578
is commonly used by researchers to measure liver function.
Other ELISA kits to measure liver function include:
- Mouse AST ELISA kit
ab263882
- Mouse Albumin ELISA kit
ab282297
- Mouse Alkaline Phosphatase ELISA kit
ab285274
ALT is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C