Abcam, ab283259, Melatonin ELISA kit

Size: 1 x 96Tests
Melatonin ELISA kit is a competitive ELISA designed to quantify Melatonin with a sensitivity of 2.5 pg/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 3 - 300 pg/ml
Key facts
Detection method:Colorimetric,
Sample types:EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:= 2.5 pg/mL,
Range:3 - 300 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Melatonin ELISA kit (ab283259) is based on the competition principal and microplate separation. Melatonin in calibrators and samples compete with a fixed amount of melatonin conjugated to horse radish peroxidase (Melatonin-HRP) for binding sites with a rabbit melatonin monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm.
The amount of Melatonin-HRP detected is inversely proportional to the amount of melatonin in a sample.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C