Abcam, ab283273, Alexa Fluor® 647 Anti-Eg5 antibody [EPR23276-52]

Size: 100µL
Rabbit Recombinant Monoclonal Eg5 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23276-52,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Eg5 protein also known scientifically as kinesin family member 11 (KIF11) plays an important role in cell mitosis. It functions mechanically as a plus-end directed motor involved in the separation of centrosomes during the early stage of cell division. Eg5 with an approximate mass of 116 kDa is responsible for the proper alignment and segregation of spindle fibers. The protein is expressed in rapidly dividing cells particularly within tissues with high turnover rates such as bone marrow and epithelium.
Biological function summary
The motor function of Eg5 is indispensable for the mitotic spindle organization. It provides the force necessary to push microtubules in opposite directions. Eg5 functions as part of a complex cellular machinery that includes proteins like dynein and other kinesins. By controlling spindle bipolarity it ensures accurate chromosome segregation during cell division preventing errors that could lead to aneuploidy.
Pathways
The action of Eg5 is fundamental in mitotic pathways including the spindle assembly checkpoint pathway. It interacts with proteins such as cyclin-dependent kinases (CDKs) which regulate its activity to ensure proper cell cycle progression. The protein's role is integral in facilitating the dynamics of the metaphase spindle checkpoint guaranteeing cell cycle fidelity.
Eg5 is linked to certain types of cancers where there is abnormal cell division. The overexpression or dysregulation of Eg5 can contribute to tumorigenesis. Additionally Eg5 interacts with proteins involved in cancer such as p53 a tumor suppressor protein directly influencing cellular proliferation and survival. Researchers target Eg5 in the development of anticancer therapies to inhibit its activity aiming to obstruct malignant cell proliferation.