Abcam, ab283552, Human TMPRSS2 ELISA Kit

Size: 1 x 96Tests
Human TMPRSS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TMPRSS2 with a sensitivity of 4.266 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - KO validated for guaranteed specificity
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.266 pg/mL,
Range:15.625 - 1000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human TMPRSS2 ELISA kit (ab283552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TMRPSS2 protein in human serum, urine, cell culture supernatants, cell culture extract and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TMPRSS2 with 4.27 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades involving physiological functions of the prostate. TMPRSS2 is understood to facilitate human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms; Proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. TMPRSS2 also proteolytically cleaves and activates human coronavirus EMC (MERS), and the fusion glycoproteins F0 of Sendai virus, human metapneumovirus, human parainfluenza 1, 2, 3, 4a and 4b viruses. TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin protein which is essential for viral infectivity. The antibodies in this product were raised to a portion of the extracellular region excluding the peptidase domain. The standard protein in this product is approximately 30% of full length TMPRSS2.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C