Abcam, ab283555, Monkey IL-2 ELISA Kit

Size: 1 x 96Tests
Monkey IL-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-2 with a sensitivity of 12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant,
Reacts with:Monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 12 pg/mL,
Range:46.88 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Monkey IL-2 ELISA kit (ab283555) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey IL-2 protein in monkey cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-2 with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states. IL-2 is both an immune stimulator and immune suppressor cytokine, which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C