Abcam, ab283887, Monkey Lipocalin-2 ELISA Kit

Size: 1 x 96Tests
Monkey Lipocalin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey Lipocalin-2 with a sensitivity of 618 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 618 pg/mL,
Range:937.5 - 60000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Monkey Lipocalin-2 ELISA Kit (ab283887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Lipocalin-2 with 618 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity, and renal development. Deficiency in Lipocalin-2 leads to increased susceptibility to bacterial infection indicating the bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Studies have shown elevated Lipocalin-2 secretion in the blood and urine after kidney injury in which its levels can be proportionally associated with the severity of prognosis, thus making Lipocalin-2 a useful biomarker for acute kidney injury.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C