Abcam, ab283966, Human Glutathione Synthetase ELISA Kit

Size: 1 x 96Tests
Human Glutathione Synthetase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Glutathione Synthetase in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 74 pg/mL,
Range:0.125 - 8 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Glutathione Synthetase ELISA Kit (ab283966) is designed for the detection of Glutathione Synthetase (GSS) in human plasma, serum, urine, saliva, milk, CSF, cell lysate and tissue samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human GSS in approximately 4 hours. A polyclonal antibody specific for human GSS has been pre-coated onto a 96-well microplate with removable strips. GSS in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human GSS, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione Synthetase (GSS) sometimes called GSS line is an enzyme that plays a central role in cellular function by catalyzing the ATP-dependent conversion of gamma-glutamylcysteine and glycine into glutathione. This enzyme has a molecular mass of about 70 kDa and exhibits high expression in the liver and red blood cells. It maintains the necessary cellular levels of glutathione a major antioxidant that protects cells from oxidative stress.
Biological function summary
GSS is responsible for the final step in glutathione biosynthesis. Acting independently GSS operates without forming a larger enzyme complex. Its enzymatic activity ensures adequate production of glutathione which regulates cellular redox homeostasis and detoxification processes. The availability of glutathione is critical for cellular respiration and immunity molecules essential for a well-functioning organism.
Pathways
The activity of GSS integrates into the gamma-glutamyl cycle and the detoxification pathway. It partners indirectly with enzymes like gamma-glutamylcysteine synthetase which precedes GSS in the glutathione biosynthesis pathway. This relationship positions GSS as an important element in sustaining normal cellular detoxification processes ensuring the neutralization of reactive oxygen species and maintaining the balance of free radicals.
Defects in GSS activity correlate with glutathione synthetase deficiency an autosomal recessive disorder that results in increased oxidative damage and metabolic acidosis. Symptoms range from hemolytic anemia to neurological complications due to impairments in oxidative stress management. The disorder links to proteins involved in antioxidant processes highlighting the importance of GSS in maintaining proper cellular function and disease prevention.