Abcam, ab283971, Human Quinine oxidoreductase ELISA Kit

Size: 1 x 96Tests
Human Quinine oxidoreductase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Quinine oxidoreductase in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 25 pg/mL,
Range:0.037 - 2.4 ng/mL,
Assay time:4h,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
The Human Quinine oxidoreductase ELISA Kit (ab283971) is designed for detection of Quinine oxidoreductase in human plasma, serum, milk, urine, saliva, cell culture, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Quinine oxidoreductase in approximately 4 hours.
A polyclonal antibody specific for human quinine oxidoreductase has been pre-coated onto a 96-well microplate with removable strips. Quinine oxidoreductase in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human quinine oxidoreductase, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Quinone oxidoreductase also known by its popular alternate name NQO1 is a flavoenzyme with a molecular mass of approximately 31 kDa. It functions mechanically by catalyzing the reduction of quinones to hydroquinones utilizing NAD(P)H as an electron donor. This enzymatic activity helps in preventing redox cycling and oxidative stress by removing reactive quinones. Quinone oxidoreductase is expressed in various tissues including liver lung and heart highlighting its role in many biological processes.
Biological function summary
Quinone oxidoreductase acts as a detoxifying enzyme. It maintains cellular protection against oxidative damage by neutralizing harmful quinones. This enzyme is not reported as part of a larger protein complex but it plays a complementary role with other phase II detoxification enzymes like glutathione S-transferase. Its protective attributes attribute to cell survival especially under oxidative stress conditions.
Pathways
Quinone oxidoreductase is integral to the oxidative stress response and xenobiotic metabolism pathways. It is closely related to proteins such as cytochrome P450 and glutathione which work together to maintain cellular redox homeostasis and metabolism of foreign compounds. This collaboration allows quinone oxidoreductase to help in converting toxic substances into less harmful forms.
Quinone oxidoreductase is implicated in cancer and neurodegenerative disorders. In cancer its overexpression can lead to chemoresistance as its detoxifying functions may neutralize chemotherapeutic agents sharing functional pathways with proteins like p53. In neurodegenerative diseases altered activity of quinone oxidoreductase influences oxidative damage a common cause in disorders such as Parkinson's disease. Its relation with proteins like PARK7 (DJ-1) highlights its role in protecting neuronal cells from oxidative stress.