Abcam, ab283982, Human CENP-B ELISA Kit

Size: 1 x 96Tests
Human CENP-B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CENP-B in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.42 ng/mL,
Range:0.625 - 40 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human CENP-B ELISA Kit (ab283982) is designed for detection of CENP-B in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CENP-B in approximately 4 hours.
A polyclonal antibody specific for human CENP-B has been pre-coated onto a 96-well microplate with removable strips. CENP-B in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CENP-B, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CENP-B also known as Centromere Protein B is a protein with a molecular weight of approximately 80 kDa. It binds to the centromeric DNA and plays a critical role in centromere formation and function. CENP-B is heavily expressed in dividing cells particularly in tissues where cell proliferation is high such as bone marrow and epithelial tissues. Researchers often use tools like anti-CENP-B antibodies and CENP-B ELISA kits to study its expression in various experimental settings.
Biological function summary
CENP-B participates in the assembly of the centromere protein complex contributing to the stability and maintenance of the centromere structure during cell division. It acts as a foundation for kinetochore nucleation ensuring proper chromosome segregation. CENP-B is involved in the formation of the CENP-A nucleosome complex important for accurate chromosomal alignment and separation.
Pathways
CENP-B is essential in the mitotic spindle assembly and chromosome segregation pathways. This protein interacts with CENP-A a variant histone which integrates into centromeric nucleosomes to initiate the formation of functional kinetochores. Additionally CENP-B interacts with CENP-C facilitating the recruitment of other centromere-associated proteins needed for chromosome dynamics during mitosis.
CENP-B is primarily associated with autoimmune disorders such as systemic sclerosis (scleroderma). Patients with these conditions often produce anti-CENP-B antibodies which serve as diagnostic markers. Moreover CENP-B's dysregulation links to chromosomal instability a contributor to cancer. Research has shown connections between CENP-B and related proteins like CENP-A in the context of chromosomal aneuploidies and malignancies.