Abcam, ab284003, Human GSTZ1 ELISA Kit

Size: 1 x 96Tests
Human GSTZ1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GSTZ1 in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 54 pg/mL,
Range:0.125 - 8 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Glutathione S-Transferase Zeta 1 (GSTZ1) ELISA Kit (ab284003) is designed for detection of GSTZ1 in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human GSTZ1 in approximately 4 hours.
A polyclonal antibody specific for human GSTZ1 has been pre-coated onto a 96-well microplate with removable strips. GSTZ1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human GSTZ1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSTZ1 also known as Glutathione S-Transferase Zeta 1 is an enzyme that plays an important role in the metabolism of various chemical compounds. It has a molecular mass of approximately 34 kDa. GSTZ1 is ubiquitously expressed in many tissues with significant levels in the liver kidney and heart. This enzyme is part of the GST family which contributes to detoxification processes by conjugating reduced glutathione to various substrates.
Biological function summary
GSTZ1 participates in the breakdown of amino acids specifically in the catabolic pathway of L-phenylalanine and L-tyrosine. The enzyme assists in the production of maleylacetoacetate and its isomerization to fumarylacetoacetate an essential step in the phenylalanine and tyrosine catabolism. GSTZ1 does not form part of a larger multi-protein complex but its function is critical in maintaining cellular homeostasis by preventing the accumulation of potentially toxic intermediates.
Pathways
GSTZ1 integrates into the metabolic pathway that includes glutathione metabolism and the phenylalanine and tyrosine degradation pathway. It interacts functionally with other enzymes like Fumarylacetoacetate hydrolase (FAH) and Phenylalanine hydroxylase (PAH) playing roles in sequential catalysis steps. These pathways are vital for maintaining amino acid balance and detoxification of byproducts within the organism.
GSTZ1 has been linked to metabolic disorders like Tyrosinemia type I and various forms of cancer. Tyrosinemia type I a disorder caused by defects in tyrosine metabolism directly implicates GSTZ1 as an important player in managing toxic intermediates. Additionally altered expression of GSTZ1 has been observed in different cancer types suggesting its potential role in tumorigenesis. FAH related through the Tyrosinemia disorder represents another key protein that GSTZ1 activity impacts highlighting its disease association.