Abcam, ab284038, Anti-SARS-CoV-2 nsp16 antibody [EPR24841-18]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for WB, Flow Cyt (Intra), ICC/IF, I-ELISA and reacts with Recombinant full length protein - SARS-CoV-2, Transfected cell line - SARS-CoV-2 samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24841-18,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:WB, ICC/IF, I-ELISA, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp16 also known as non-structural protein 16 is an important enzyme in the replication and transcription machinery of the virus. It exhibits 2'-O-methyltransferase activity and has a molecular mass of approximately 33 kDa. Nsp16 is expressed in host cells once the virus infects the cell and translates its polyproteins. Its main role is to modify the cap structure of viral mRNA which helps in avoiding host immune detection and promoting efficient viral replication.
Biological function summary
The function of nsp16 centers around its interaction with nsp10 forming a stable 2'-O-methyltransferase complex. This enzyme complex is responsible for the methylation of the ribonucleic acid cap at the first nucleotide. This cap modification is necessary for the stability of the viral mRNA and its ability to be recognized by the host's cellular machinery as native mRNA which prevents antiviral defenses from targeting the virus. The methylation activity of nsp16 is highly specific requiring both nsp10 and nsp16 to function together effectively.
Pathways
Nsp16 plays an integral role in the viral replication-transcription complex (RTC) pathway. This pathway is essential for the synthesis of viral genomic and subgenomic RNAs facilitating the life cycle of SARS-CoV-2. The RTC includes other viral proteins like nsp12 which acts as the RNA-dependent RNA polymerase. These interactions ensure the replication fidelity and quantity necessary for viral protein production and spreading within the host organism.
Nsp16 is connected to COVID-19 the disease caused by SARS-CoV-2 infection. Its role in immune evasion makes it a target for antiviral drug development as disabling the nsp16 function could make the virus more susceptible to host immune responses. nsp16's cooperation with nsp10 is also significant in this context as disrupting their interaction might prevent the virus from effectively replicating offering a potential therapeutic strategy to combat COVID-19.