Abcam, ab284039, Anti-SARS-CoV-2 nsp5 antibody [EPR24844-87]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for I-ELISA and reacts with SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24844-87,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 nsp5 also known as 3C-like protease or Mpro plays an important mechanical role in the life cycle of the virus. It is a cysteine protease with a mass around 33.8 kDa responsible for processing the viral polyprotein into functional units essential for viral replication. Nsp5 carries out cleavage at specific sites on the polyprotein enabling the viral replication machinery. This protein is expressed in the infected host cells where it facilitates the maturation of viral components.
Biological function summary
The 3C-like protease facilitates efficient viral replication by precisely cleaving the polyprotein into individual non-structural proteins. Nsp5 does not function alone; it is part of the replicase-transcriptase complex that includes other non-structural proteins essential for virus propagation. Its activity is critical for preparing the molecular toolkit the virus needs for replicating its genome and assembling new virions within the host cell.
Pathways
The nsp5 protease is deeply involved in the viral replication and transcription pathways. This process links it to critical elements such as nsp12 the RNA-dependent RNA polymerase showing a direct relationship through sequential proteolytic processing. The treatment of COVID-19 often targets this pathway as inhibiting nsp5 can disrupt viral replication highlighting its significance in antiviral strategies.
Nsp5 is a primary target in the context of COVID-19 the disease caused by SARS-CoV-2 infection. Because of its central role in viral replication it is a focal point in drug development efforts aimed at COVID-19 treatment. The interactions between nsp5 and structural proteins like the spike protein highlight its importance as disrupting these interactions can potentially reduce viral infectivity and pathogenicity within the host.