Abcam, ab284133, Alexa Fluor® 647 Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

Size: 100µL
Rabbit Recombinant Monoclonal LAMP2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4207(2),
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMP2A also known as lysosome-associated membrane protein 2A functions as a lysosomal marker and plays a role in the lysosome size and structure regulation. Its molecular weight is approximately 45 kDa. This protein is expressed mainly in tissues with high metabolic activity such as liver kidney and heart. LAMP2A is an integral component of the lysosomal membrane and is important for the proper function and maintenance of lysosomes which are cellular organelles involved in the degradation and recycling of macromolecules.
Biological function summary
LAMP2A facilitates chaperone-mediated autophagy (CMA) a specific mechanism for the selective degradation of proteins. It is not part of a larger complex but rather acts independently within the lysosomal membrane. This transport functions through recognizing cytosolic proteins containing a KFERQ-like motif which are then translocated into the lysosome for degradation. LAMP2A upholds cellular homeostasis and is important in adapting to cellular stress by facilitating the turnover of damaged or unnecessary proteins.
Pathways
LAMP2A is intimately involved in the CMA pathway. This pathway is important for regulating protein quality control often linked with other autophagic processes. LAMP2A also interacts with the heat shock cognate protein (HSC70) which assists in unfolding and transporting substrate proteins to lysosomes. Through these interactions LAMP2A helps maintain cellular proteostasis which is essential for cellular function and survival especially during stress conditions.
LAMP2A is connected to lysosomal storage disorders such as Danon disease. This disease is characterized by the accumulation of autophagic vacuoles due to defects in lysosomal function. LAMP2A mutations alter lysosomal degradation capabilities leading to severe cardiomyopathy and myopathy. Additionally LAMP2A has implications in neurodegenerative diseases like Parkinson's disease due to its role in protein degradation pathways. In these contexts malfunction or altered expression of LAMP2A can disrupt cellular clearance processes contributing to pathogenesis along with proteins such as alpha-synuclein.