Abcam, ab284856, Anti-OSTF1 antibody [EP15457] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Monoclonal OSTF1 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP15457,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WB, ICC/IF, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284856 is the carrier-free version of
ab202901
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OSTF1 also known as Osteoclast-Stimulating Factor 1 is an important protein involved in the regulation of bone resorption. It has a molecular weight of approximately 60 kDa. OSTF1 is widely expressed in bone tissues where it influences the activity of osteoclasts. Researchers have identified it as a cytoplasmic protein that plays a role in signaling processes. OSTF1 functions as an adapter protein linking other proteins involved in cellular functions related to bone metabolism.
Biological function summary
OSTF1 interacts with cytoskeleton-associated proteins to enhance osteoclast activity. It coordinates the assembly of protein complexes essential for cell movement and actin cytoskeleton organization. OSTF1 is known to engage in complex formations with proteins such as Src a tyrosine kinase that facilitates its regulatory activities in osteoclasts. This interaction highlights OSTF1's role in the dynamics of cell adhesion and mobility which are important for bone remodeling processes.
Pathways
OSTF1 plays a role in the RANK/RANKL/OPG signaling pathway an important pathway for bone homeostasis. It partners with the Src protein in this pathway influencing osteoclast differentiation and activation. Besides OSTF1 is linked to the integrin signaling pathway through its interactions with cytoskeleton components which is equally significant for cell adhesion and survival. By mediating these interactions OSTF1 helps modulate the balance between formation and breakdown of bone matrix.
OSTF1 has associations with osteoporosis and Paget’s disease of bone. In osteoporosis the deregulation of OSTF1 influences abnormal osteoclast activity leading to increased bone resorption and fragility. In Paget’s disease OSTF1 may interact with other proteins like TNF receptor-associated factor 6 (TRAF6) affecting the excessive breakdown and formation of bone. The involvement of OSTF1 in these conditions makes it a target of interest for therapeutic interventions in bone diseases.