Abcam, ab284859, Anti-GnRH antibody [EPR24529-70] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GnRH antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24529-70,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Data from recombinant overexpression of GnRH demonstrating reactivity in WB, ICC, IP and Flow Cyt (intracelluar) is available.

Product details:
ab284859 is the carrier-free version of
ab281844
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gonadotropin-releasing hormone (GnRH) also referred to as luteinizing hormone-releasing hormone (LHRH) or gonadotropin peptide is a small peptide hormone with a molecular weight of approximately 1.2 kDa. GnRH is chiefly expressed in the hypothalamic neurons located in the pre-optic area of the brain. It is synthesized as a preprohormone and undergoes cleavage to become its active form. GnRH plays an important role in the regulation of reproductive functions acting as an important regulator of the release of pituitary gonadotropins.
Biological function summary
GnRH influences reproduction by stimulating the anterior pituitary gland to secrete two essential gonadotropins: luteinizing hormone (LH) and follicle-stimulating hormone (FSH). These gonadotropins further act on the gonads to promote steroidogenesis and gametogenesis essential processes for fertility. The GnRH peptide hormone does not typically form part of a larger protein complex. Instead it interacts directly with specific receptors on the surface of anterior pituitary cells to carry out its functions.
Pathways
The gonadotropin peptide acts within the hypothalamic-pituitary-gonadal (HPG) axis an integral pathway for maintaining reproductive health and hormonal balance. This pathway involves GnRH LH FSH and steroid hormones like estradiol and testosterone. In the HPG axis GnRH release is subjected to feedback mechanisms from the steroid hormones ensuring the fine-tuning of reproductive hormone levels. Additionally this peptide is part of the neuroendocrine control pathway interacting with other neuropeptides like kisspeptin which modulates its secretion thereby influencing overall reproductive function.
Dysfunctions in GnRH signaling can lead to conditions such as hypogonadotropic hypogonadism and precocious puberty. These disorders result from insufficient or excessive release of gonadotropins due to impaired GnRH action. In hypogonadotropic hypogonadism there is a failure in stimulation of LH and FSH leading to inadequate sex steroid production and subsequent infertility or delayed puberty. Conversely excessive GnRH activity may result in precocious puberty where early activation of the reproductive axis occurs. Anti-GnRH therapies aim to modulate these dysfunctions aligning related proteins like kisspeptin and neurokinin B with therapeutic approaches.