Abcam, ab284995, Anti-FRZB antibody [EPR23904-127] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FRZB antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23904-127,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284995 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse/Rat FRZB. The recommended pair for sandwich ELISA is:
Capture: ab284995, Mouse/Rat FRZB Capture Antibody (unconjugated)
Detector: ab285101, Mouse/Rat FRZB Detector Antibody (unconjugated)
The reference range value is 140.63 pg/mL - 9000 pg/mL.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FRZB also known as secreted frizzled-related protein 3 (SFRP3) functions as a modulator of Wnt signaling pathways. This protein has a mass of approximately 36 kDa and serves as an antagonist to the Wnt proteins by binding to them preventing their interaction with cell surface receptors. FRZB is expressed in several tissues with notable levels in cartilage bone and the heart.
Biological function summary
The modulation of Wnt signaling by FRZB plays a significant role in embryonic development and tissue homeostasis. FRZB is not part of a protein complex but has important interactions with other components of the signaling cascade. It influences cell differentiation proliferation and apoptosis which are critical processes during development and tissue repair.
Pathways
FRZB influences both Wnt/β-catenin and non-canonical Wnt pathways. Its interaction with these pathways regulates signal transmission that affects cellular behaviors. Related proteins include Wnt ligands and frizzled receptors which are central to these signaling mechanisms.
Abnormalities in FRZB expression or function show a connection to osteoarthritis and certain cancers. In osteoarthritis FRZB interacts with Wnt proteins which contribute to the degradation of cartilage. In cancer dysregulation of Wnt signaling involving FRZB can lead to unchecked cell proliferation. Understanding its role in these diseases can aid in developing therapeutic strategies.