Abcam, ab285235, Mouse Troponin I ELISA Kit

Size: 96Test
Mouse Troponin I ELISA Kit is a sandwich ELISA designed to quantify Mouse Troponin I with a sensitivity of 18.75 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 31.25 - 2000 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Other biological fluids,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 18.75 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Troponin I ELISA Kit ab285235 is a sandwich ELISA to measure Mouse Troponin I in serum, plasma, other biological fluids with a sensitivity of 18.75 pg/ml.
How the assay works
The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse Troponin I. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for mouse Troponin I and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of human Troponin I. The concentration of mouse Troponin I in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Troponin I ELISA Kit ab285235 protocol summary
1. Add standards and samples to appropriate wells.Incubate
2. Wash
3. Add Biotinylated Detection Antibody. Incubate
4. Wash
5. Add HRP Conjugate. Incubate
6. Wash
7. Add Substrate Reagent. Incubate
8. Add stop solution and read at 450 nm
This product is manufactured by BioVision, an Abcam company and was previously called E4736 Troponin I (Mouse) ELISA Kit. E4736-100 is the same size as the 96 test size of ab285235.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols