Abcam, ab285250, Human Collagen Type I ELISA Kit

Size: 96Test
Human Collagen Type I ELISA kit (colorimetric) is a sandwich ELISA designed to quantify human collagen type I (COL1A1) with a sensitivity of 0.188 ng/mL. - Wide dynamic range – quantifies 0.313–20 ng/mL - Compatible with multiple sample types – works with serum, plasma, tissue homogenates, cell culture supernatants, cell lysates, and other biological fluids - Assay platform – microplate (12 x 8 well strips) for easy setup and reliable results
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids, Cell culture supernatant, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich,
Sensitivity:< 0.188 ng/mL,
Range:0.313 - 20 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human Collagen Type I ELISA Kit ab285250 is a sandwich ELISA kit to measure human collagen type I (COL1A1) with a sensitivity of 0.188 ng/mL.
How the assay works
Human Collagen Type I ELISA Kit ab285250 uses a pair of antibodies each specific to different epitopes at the target of interest: the capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Human Collagen Type I ELISA Kit ab285250 protocol summary
1. Add standards or samples to the wells, incubate 2 hrs
2. Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
3. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
4. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
5. Add 50 µl of Stop Solution per well. Read at 450 nm immediately
How other researchers are using Human Collagen Type I ELISA Kit ab285250
Human Collagen Type I ELISA Kit ab285250 has been used to study USP37 regulation of c-Myc to promote keloid fibroblast proliferation and collagen production
References:
Guangdong Feng et al. 2023, PMID: 36333840
Related and recommended products
Human Collagen Type I ELISA Kit ab285250 is commonly used to study tissue structure and function.
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.