Abcam, ab285259, Mouse/Rat Triiodothyronine (T3) ELISA Kit

Size: 96Test
Mouse/Rat Triiodothyronine (T3) ELISA Kit is a competitive ELISA designed to quantify Mouse/Rat Triiodothyronine (T3) with a sensitivity of 0.186 ng/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.5 - 1.5 ng/ml
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Rat, Mouse,
Assay type:Competitive,
Sensitivity:= 0.186 ng/mL,
Range:0.5 - 1.5 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The mouse/rat Triiodothyronine (T3) kit (ab285259, K7422) is a solid phase competitive ELISA Kit. The samples, and T3 enzyme conjugate are added to the wells coated with anti-T3 polyclonal antibody. T3 in the sample competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Triiodothyronine (T3) also known as liothyronine is a thyroid hormone with a molecular mass of approximately 651.97 Da. It forms when enzymes called deiodinases especially in the liver and kidneys remove an iodine from thyroxine (T4). T3 is mainly expressed in cells of tissues with high metabolic activity including the heart liver and brain. In plasma T3 travels bound to proteins like thyroxine-binding globulin which helps maintain its stable concentration.
Biological function summary
T3 plays a central role in regulating metabolic processes and energy homeostasis. It acts on nearly every cell in the body influencing protein synthesis increasing basal metabolic rate and consequently affecting development growth and metabolism. T3 exerts its effects by binding to nuclear thyroid hormone receptors forming a hormone-receptor complex that regulates gene expression. This hormone engages in crosstalk with other signaling molecules linking it to broader metabolic networks.
Pathways
T3 integrates into the thyroid hormone signaling pathway influencing various metabolic and physiological functions. This pathway includes the conversion of T4 to T3 by iodothyronine deiodinases. T3 also interacts with the PI3K/Akt pathway an important signaling cascade involved in cellular survival and growth. Proteins like thyroid receptor alpha (TRα) and beta (TRβ) are vital in mediating the effects of T3 as they serve as binding sites for the hormone influencing the pathway's downstream function.
Alterations in T3 levels associate with conditions such as hyperthyroidism and hypothyroidism. Hyperthyroidism results from excess T3 leading to symptoms like increased heart rate and weight loss while hypothyroidism stems from T3 deficiency causing fatigue and weight gain. T3 plays a role in Graves’ disease an autoimmune disorder that can cause hyperthyroidism with thyroid-stimulating hormone receptor antibodies influencing the overproduction of the hormone. Dysregulation of T3 levels also impacts the cardiovascular system through its connection with proteins involved in heart function potentially contributing to heart disease.