Abcam, ab286057, Recombinant human FBP1 protein (Active)

Size: 10µg / 50µg
Recombinant human FBP1 protein (Active) is a Human Full Length protein, in the 1 to 388 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is ≥ 1 U/mg.One unit is the amount of enzyme that will hydrolyze 1.0 μmole of fructose 1,6-bisphosphate to fructose 6-phosphate at pH 8.0 and 37°C for 30 minutes.,
Accession:P09467,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in deionized water to a final concentration of 1.5 µg/ml. The reconstituted enzyme can be stored at 4°C for up to 2 weeks. For long term storage, aliquot and store at -70°C.,
Storage buffer:Constituents: PBS, 8% Trehalose

Product details:
This product is manufactured by BioVision, an Abcam company and was previously called P1269 Fructose 1,6-bisphophatase 1, Active, Human Recombinant. P1269-50 is the same size as the 50 μg size of ab286057.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FBP1 also known as fructose-16-bisphosphatase 1 is an enzyme with a molecular mass of approximately 37 kDa. It plays a mechanical role in catalyzing the conversion of fructose-16-bisphosphate to fructose 6-phosphate in the gluconeogenesis pathway. This enzyme expresses primarily in the liver but also in kidneys and muscles reflecting its key role in glucose metabolism.
Biological function summary
The function of FBP1 involves maintaining glucose homeostasis which is essential for energy balance. FBP1 is part of the gluconeogenesis pathway a process that synthesizes glucose from non-carbohydrate sources. This enzyme acts independently and does not usually form part of larger protein complexes but its activity significantly impacts liver metabolism and energy production in low-glucose conditions.
Pathways
FBP1 finds its primary involvement in the gluconeogenesis pathway. This roles complements those of other enzymes such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase which also contribute to glucose synthesis. FBP1's activity supports glucose production providing glucose for bloodstream circulation when dietary intake is low.
FBP1's function directly relates to conditions such as fructose 16-bisphosphatase deficiency and has implications in type 2 diabetes. Deficient FBP1 can lead to life-threatening hypoglycemia demonstrating the need for energy regulation by this enzyme. Its regulation intersects with insulin signaling pathways with proteins like insulin receptor substrate (IRS) playing roles in modulating its activity in metabolic disorders.