Abcam, ab286179, Anti-SARS spike glycoprotein+COVID-19 Spike Protein antibody [CR3022]

Size: 200µg
Human Recombinant Monoclonal SPIKE antibody. Suitable for ELISA and reacts with SARS-CoV-2, SARS-CoV samples. Cited in 1 publication.
Key facts
Host species:Human,
Clonality:Monoclonal,
Clone number:CR3022,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:SARS-CoV-2, SARS-CoV,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This product is manufactured by BioVision, an Abcam company and was previously called A2103 Anti-CoV-2 & SARS-CoV S1 Antibody (Clone CR3022). A2103-200 is the same size as the 200 μg size of ab286179.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS spike glycoprotein also known as the COVID-19 spike protein is an important target in the study of coronaviruses. This protein with a mass of approximately 180–200 kDa plays an essential role in the virus's ability to infect host cells. Expressed on the surface of the virus the spike protein facilitates the viral entry by binding to the angiotensin-converting enzyme 2 (ACE2) receptors present on human cells. Through this interaction the spike protein initiates the fusion of the viral membrane with the host cell membrane enabling the virus to infiltrate the host.
Biological function summary
The spike protein performs several significant functions beyond its role in viral entry. It forms part of a larger viral structure together with other proteins that maintain the virus's shape and functionality. The spike protein is integral in host immune system activation as it contains multiple antigenic sites that elicit an immune response. These antigenic sites become targets for antibodies that neutralize the virus. Researchers often focus on this aspect to develop vaccines and therapeutic interventions as it determines the body's capacity to fight off the infection.
Pathways
The spike protein impacts both viral lifecycle and immune response pathways. During the viral lifecycle it is critical for initiating receptor-mediated endocytosis a pathway by which the virus gains cellular entry. Additionally its interaction with ACE2 links it to the renin-angiotensin system (RAS) pathway impacting cardiovascular regulation. Proteins such as TMPRSS2 which assists in priming the spike protein for membrane fusion also play a significant role within these pathways. Understanding these mechanisms contributes to the development of strategies to inhibit virus action and spread.
The spike protein is closely associated with COVID-19 the disease caused by the SARS-CoV-2 virus. Its function directly relates to the virus's ability to cause acute respiratory distress syndrome (ARDS) a severe condition common in critical COVID-19 patients. Researchers also study its potential links to cardiovascular issues as seen with co-expression and interaction with ACE2 in heart tissue. Furthermore the immune response triggered by the spike protein leads to the production of anti-spike protein antibodies which are vital components of serological tests for COVID-19 diagnostics and monitoring.