Abcam, ab28666, Anti-ECM29 antibody

Size: 100µL
Rabbit Polyclonal ECM29 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ECPAS aa 1800 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ECPAS aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q5VYK3

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.05% Sodium azideConstituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ECM29 also known as proteasome-associated protein ECM29 homolog is a multi-domain protein with a molecular mass of approximately 209 kDa. The protein acts as an adaptor that links molecular chaperones to the proteasome. Highly expressed in tissues with high proteasome activity ECM29 mainly locates in the cytosol. The structure of ECM29 suggests it interacts with the proteasomal regulatory particle altering its conformation and functional state.
Biological function summary
ECM29 impacts protein degradation by stabilizing the interaction between the 19S regulatory particle and the 26S proteasome core complex. It plays a role in ensuring the correct assembly of the proteasome which is essential for the ubiquitin-proteasome system. This system is responsible for the majority of protein turnover in the cell impacting various cell cycle processes. ECM29's association with other proteasome components like Rpn1 and Rpn2 signifies its role in protein homeostasis.
Pathways
ECM29 integrates into the ubiquitin-proteasome pathway and cell cycle regulation mechanisms. It coordinates with proteins such as ubiquitin and Rpn10 to manage protein degradation. Its involvement in NAD(P)H pathway regulation has been noticed where it potentially influences cellular responses to stress by modulating proteasome activity under different conditions.
Variations in ECM29 expression and regulation implicate it in neurodegenerative diseases such as Alzheimer's. Aberrant proteasome function connected to ECM29 can disrupt protein clearance contributing to protein aggregation observed in Alzheimer's. Additionally ECM29 interacts with SYT7 influencing synaptic function and potentially impacting neuropathologies. Understanding ECM29's function provides deeper insight into therapeutic targets for managing proteasome-related disorders.