Abcam, ab2871, Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control

Size: 200µL
Mouse Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Sheep ATP1A1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:M7-PB-E9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, IHC-P, ICC/IF, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Sheep ATP1A1. The exact immunogen used to generate this antibody is proprietary information.P04074,
Epitope:This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium / potassium ATPase.,
Specificity:Detects pan alpha sodium / potassium ATPase. The antibody binds all alpha subunits in sheep, dog, pig, human and chicken. It does not bind alpha 1 or 2 in rat, but does bind alpha 3.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan ATPase Alpha also known as 'alpha pan' or 'pb alpha' is a protein complex with an approximate molecular mass of 100 kDa. It is part of a larger family of ATPases which hydrolyze ATP to ADP releasing energy used in various cellular processes. The protein is expressed in diverse tissues in the body reflecting its essential role in cellular metabolism. It consists of multiple subunits that work together to maintain the proton gradient across membranes important for energy homeostasis.
Biological function summary
Different subunits of ATPase Alpha are important for cellular energy metabolism and are part of a larger enzymatic complex. This complex facilitates proton translocation in cellular organelles contributing to energy conversion processes. Through ATP hydrolysis this protein helps maintain ion gradients which are employed by cells for multiple functions such as nutrient uptake and pH balance. The machinery provided by ATPase Alpha is vital for cell survival and function.
Pathways
The activity of ATPase Alpha integrates into significant metabolic pathways such as oxidative phosphorylation and glycolysis. It is central in energy transduction providing ATP required for many cellular activities. The protein modulates activity in conjunction with other components like the cytochrome c oxidase complex in the mitochondrial electron transport chain playing a role in producing the cell's energy currency. Pan ATPase Alpha's ability to interface with glycolysis allows cells to effectively manage energy production and consumption.
Abnormal levels of ATPase Alpha are linked to conditions such as metabolic syndrome and neurodegenerative diseases. In metabolic syndrome dysregulation of ATPase Alpha may contribute to impaired energy metabolism and insulin resistance. Additionally alterations in its function can relate to neurodegenerative disorders where mitochondrial dysfunction is prevalent. In these diseases ATPase Alpha interacts with other key proteins such as amyloid precursor protein in Alzheimer's disease indicating its possible role in disease pathogenesis and progression.