Abcam, ab287893, Anti-EZH2 antibody [EPR24902-112]

Size: 20µL / 100µL / 1mL
Knockout Tested Rabbit Recombinant Monoclonal KMT6 / EZH2 antibody. Suitable for WB, I-ELISA and reacts with Human, Mouse, Synthetic peptide samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24902-112,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:I-ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Enhancer of Zeste Homolog 2 (EZH2) is an enzymatic component of the polycomb repressive complex 2 (PRC2). Known also as KMT6 EZH2 functions as a histone methyltransferase catalyzing the tri-methylation of histone H3 at lysine 27 (H3K27me3). This methylation activity is important for gene silencing. EZH2 has a molecular mass of approximately 85 kDa and is expressed in various tissues with higher expression often observed in embryonic stem cells and certain adult tissues such as prostate and breast tissue.
Biological function summary
EZH2 plays an important role in chromatin remodeling and transcriptional repression through its integration within the PRC2 complex which includes proteins such as SUZ12 and EED. This complex is involved in maintaining the transcriptional silencing of genes essential for development and differentiation. EZH2's activity regulates cell fate decisions and helps preserve stem cell pluripotency through repression of lineage-specific genes.
Pathways
EZH2 integrates into mechanisms often seen in the regulation of the Wnt and Hedgehog signaling pathways. These pathways are central to developmental processes and cell proliferation. In the Wnt pathway EZH2 interacts with proteins such as β-catenin impacting gene expression involved in cell growth. Similarly within the Hedgehog pathway EZH2 may influence gene regulation through its interaction with GLI transcription factors again affecting cellular proliferation and tissue patterning.
EZH2 has strong associations with cancer particularly in conditions like prostate cancer and lymphomas. Overexpression or mutations in EZH2 contribute to tumorigenesis by promoting aberrant silencing of tumor suppressor genes. In the context of lymphomas mutations in EZH2 can cooperate with alterations in associated proteins like BCL2 to drive oncogenesis. Furthermore therapeutic interventions targeting EZH2 are under investigation highlighting its relevance in disease progression and potential treatment.