Abcam, ab288304, Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NOL3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25182-11,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat, Mouse,
Applications:ICC/IF, IHC-P, WB, IP, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab288304 is the carrier-free version of
ab288295
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nucleolar protein 3 (NOL3) also known as ARC (apoptosis repressor with caspase recruitment domain) is a multifunctional protein involved in apoptosis regulation and cellular stress responses. It has a molecular mass of approximately 26 kDa. NOL3 is expressed in various tissues but it displays high levels in the heart skeletal muscles and neurons. Its expression suggests a protective role in these tissues where it interacts with various cellular components to prevent apoptosis.
Biological function summary
NOL3 contributes significantly to cell survival and apoptosis inhibition by interacting with pro-apoptotic proteins to block their function. It acts as a safeguard against external stressors that can trigger cell death. As part of its role in apoptosis regulation NOL3 engages in complex formation with other proteins to modulate apoptotic pathways. For instance it binds directly to caspases preventing their activation and further propagation of cell death signals.
Pathways
NOL3 plays a critical role in the apoptosis and stress response pathways. It participates in the intrinsic apoptosis pathway by inhibiting the activation of key apoptotic enzymes while also interacting with proteins such as Bcl-2 and Bax to further regulate cell survival. The involvement of NOL3 in stress response pathways highlights its function in promoting cellular resilience in challenging conditions often functioning together with heat shock proteins that assist in protein folding and stress recovery.
NOL3 has been implicated in neurodegenerative diseases and cardiac dysfunction. In particular its dysregulation is associated with conditions like Alzheimer's disease where it might influence neural cell survival. Furthermore NOL3 interacts with amyloid precursor protein (APP) which has a role in Alzheimer's pathology. In the context of cardiac diseases altered NOL3 expression may influence cardiac hypertrophy and heart failure potentially interacting with proteins such as caspase-8 in these processes.