Abcam, ab288710, Human SGIP1 knockout SK-N-FI cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
SGIP1 KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:SK-N-FI,
Species or organism:Human,
Tissue:Bone marrow,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Disease:Neuroblastoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SGIP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SH3-domain GRB2-like endophilin B1 interacting protein 1 commonly known as SGIP1 weighs around 118 kDa. This protein is involved in clathrin-mediated endocytosis processes. SGIP1 interacts with adaptor proteins playing a role in endocytosis by modulating the internalization of membrane components into the cell. Researchers have identified its expression mainly in the brain especially in neurons and it has been observed to a lesser extent in other tissues.
Biological function summary
SGIP1 plays a role in neural activities by contributing to the regulation of synaptic vesicle recycling. It assists in neurotransmitter uptake influencing synaptic transmission efficiency. SGIP1 interacts with components of endocytic vesicle formation forming part of a larger protein complex that includes clathrin and dynamin. This complex is necessary for the vesicle scission and cargo selection during endocytosis.
Pathways
SGIP1 is involved in the clathrin-mediated endocytosis pathway essential for recycling synaptic vesicles and maintaining synaptic function. It operates alongside proteins like dynamin 1 a GTPase important for vesicle fission. In the context of synaptic function SGIP1 engages with pathways connected to neurotransmitter uptake impacting neuronal communication and plasticity.
SGIP1 has been associated with neurological conditions such as schizophrenia and Alzheimer's disease. Changes in its normal function may influence synaptic vesicle recycling leading to synaptic dysfunction observed in these disorders. The protein dysbindin-1 known for its connection to schizophrenia interacts with SGIP1 indicating potential overlap in their pathological roles. Understanding these interactions might provide insight into novel therapeutic strategies for these diseases.