Abcam, ab288715, Anti-S100A8 + S100A9 antibody [RM1038]

Size: 20µL / 100µL / 1mL
Anti-S100A8 + S100A9 antibody [RM1038] (ab288715) is a rabbit recombinant multiclonal antibody detecting S100A8 + S100A9 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:RM1038,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:IP, ICC/IF, IHC-P, WB, Flow Cyt (Intra), IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-S100A8 + S100A9 antibody [RM1038] (ab288715) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of S100A8 + S100A9?
Anti-S100A8 + S100A9 [RM1038] (ab288715) specifically detects a band for S100A8 + S100A9 (UniProt: P06702) at a molecular weight of 13kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S100A8 and S100A9 often known together as calprotectin are proteins that form a heterodimer and function as a complex. Each protein has a mass of approximately 10.8 kDa for S100A8 and 13.2 kDa for S100A9. These proteins express mainly in neutrophils and monocytes acting as calcium-binding proteins. Their expression can modulate various cellular functions like migration and inflammation responses.
Biological function summary
Calprotectin serves as an important player in the immune response. This complex possesses antimicrobial properties and contributes to the body's defense by modulating inflammation. It binds to zinc and manganese to deprive pathogens of essential nutrients an action called nutrient bioavailability restriction. In immune cells calprotectin's presence signals the activation and recruitment of further immune components.
Pathways
S100A8 and S100A9 influence the NF-kB signaling pathway which governs immune responses and inflammation. Their function affects other pathways such as MAPK by mediating pro-inflammatory cytokines' production and release. These proteins often engage with Toll-like receptors impacting the innate immune system and contributing to the body's defensive mechanisms against pathogens.
High levels of calprotectin are associated with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. Its involvement in these conditions makes it a valuable biomarker. Calprotectin often interacts with other inflammatory molecules like TNF-alpha an important factor in propagating inflammation. The calprotectin ELISA test kit plasma calprotectin immunoassay and similar tools can aid in diagnosing and monitoring these conditions providing insight into disease progression and response to treatment.