Abcam, ab289830, Ovalbumin (OVA) ELISA Kit

Size: 100Test
Ovalbumin (OVA) ELISA Kit is a sandwich ELISA designed to quantify Ovalbumin (OVA) with a sensitivity of 0.469 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.781 - 50 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum, Other biological fluids,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 0.469 ng/mL,
Range:0.781 - 50 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Ovalbumin (OVA) ELISA Kit ab289830 is a sandwich ELISA to measure Ovalbumin (OVA) in serum, plasma, cell culture supernatant, other biological fluids with a sensitivity of 0.469 ng/ml.
How the assay works
The density of color is proportional to the amount of Ovalbumin captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Ovalbumin (OVA) ELISA Kit ab289830 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ovalbumin also known as egg albumin is a significant protein found in egg white representing about 54% of the protein content in this part of the egg. It has a molecular weight of approximately 45 kDa. This protein is synthesized in the oviduct of birds and deposited in the egg white where it fulfills several roles. Ovalbumin is a glycoprotein meaning it has carbohydrate groups attached to the polypeptide chain. Researchers commonly use it as a model antigen in immunology studies and as a standard in protein markers because of its known properties.
Biological function summary
Ovalbumin functions as a storage protein in eggs providing essential amino acids for the developing embryo. This protein also plays a role in binding metal ions and vitamins acting as a nutrient reservoir. It is not part of a complex existing as a single polypeptide chain. Ovalbumin contributes to the regulation and transport of metabolic compounds due to its ability to bind ions. These properties make it useful in various biological studies and industrial applications.
Pathways
Four significant pathways involve the role of ovalbumin in living organisms. These include the regulation of protein adsorption and the nutrient reservoir activity pathway. Ovalbumin's ability to serve as a nutrient source directly relates to these pathways ensuring proper nourishment during embryo development. It operates independently of other proteins in these pathways emphasizing its role in providing and regulating nutrients without the aid of additional complexes.
Ovalbumin has connections to some allergic responses particularly in individuals with egg allergies. It acts as an allergen capable of provoking immune reactions. Ovalbumin lacks a direct connection to specific diseases beyond allergies. However understanding its interaction with immune system proteins like immunoglobulin E (IgE) is important as this is the primary mediator in allergic responses. This link makes ovalbumin important for research into allergy mechanisms and the development of hypoallergenic variants.