Abcam, ab289901, Mouse, Rat Kallikrein 14 ELISA Kit

Size: 1 x 96Tests
Mouse, Rat Kallikrein 14 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse, Rat Kallikrein 14 with a sensitivity of 2.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Rat, Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:>= 2.58 pg/mL,
Range:10.938 - 700 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse, Rat Kallikrein 14 ELISA Kit (ab289901) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse and Rat Kallikrein 14 in cell culture, plasma and serum samples. It uses our proprietary SimpleStep ELISA
technology. Quantitate Mouse and Rat Kallikrein 14 with 2.58 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Kallikrein 14 (KLK14) a serine protease is involved in various proteolytic activities. It has a molecular mass of approximately 25 kDa. KLK14 is part of the kallikrein-related peptidase family and is expressed in diverse tissues including skin prostate and brain. This protein is also known by the alternate name hK14. The expression pattern of KLK14 suggests its involvement in local tissue-specific functions.
Biological function summary
KLK14 influences processes like skin desquamation and seminal plasma liquefaction. It plays a significant role in the regulation of extracellular matrix components and participates in activating protein precursors. Though it functions as an individual protease KLK14 interacts with other kallikreins in a possible cascade-like manner indicating complex interplay between these proteins.
Pathways
KLK14 is key in processes involving the kallikrein-kinin system and the activation of certain growth factors. It can cleave and activate other proteins such as renin-angiotensin system components influencing blood pressure regulation. KLK14 operates within this intricate network alongside proteins like KLK1 and other kallikreins that have complementary roles in physiological pathways.
KLK14 has been linked to prostate cancer and skin-related conditions such as atopic dermatitis. Overexpression of KLK14 correlates with prostate cancer progression suggesting its involvement in tumorigenesis. It is also associated with other kallikreins like KLK3 (PSA) in cancer influencing disease development and prognosis. In skin disorders KLK14 may affect processes like inflammation and barrier function revealing its therapeutic target potential.