Abcam, ab300221, Human PCAF knockout U2-OS cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
PCAF KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. KO cell line generated by CRISPR/Cas9 system. Individual gRNAs were subcloned into pSpCas9 (BB)-2A-Puro and then stably transfected into U2OS cells. Transfected cells were selected by puromycin. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:U-2 OS,
Species or organism:Human,
Tissue:Bone,
Form:LiquidSee storage information,
Knockout validation:Western blot,
Mutation description:KO cell line generated by CRISPR/Cas9 system. Individual gRNAs were subcloned into pSpCas9 (BB)-2A-Puro and then stably transfected into U2OS cells. Transfected cells were selected by puromycin.,
Disease:Osteosarcoma

Product details:
For more information about this cell line please see publication: PMID: 31753913.
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PCAF, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-A few days, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KAT2B also known as PCAF (p300/CBP-associated factor) is a histone acetyltransferase protein with a mass of approximately 94 kDa. It plays a role in chromatin remodeling by acetylating lysines on histone tails which facilitates transcriptional activation. KAT2B is expressed ubiquitously but higher expressions occur in tissues with active transcription such as the brain and testes. KAT2B/PCAF can also interact with transcription regulators adding another layer to its role in gene expression regulation.
Biological function summary
KAT2B/PCAF participates in modifying chromatin structure and impacting transcription. It forms part of various complexes most notably a complex with other proteins like p300 and CBP which have acetyltransferase activity too. Through modifying histones KAT2B/PCAF influences gene expression patterns critical for cell cycle regulation and differentiation. It also acetylates non-histone proteins playing roles in diverse biological processes beyond transcription.
Pathways
KAT2B/PCAF integrates into the regulation of cell cycle and apoptosis pathways. One of the major pathways it involves in is the p53 signaling pathway which is important for cell cycle arrest and apoptosis in response to DNA damage. KAT2B/PCAF works with proteins like p53 and HDACs (histone deacetylases) to fine-tune the balance between acetylation and deacetylation of histones and other proteins influencing cell cycle progression and cellular response to stress signals.
KAT2B/PCAF has associations with cancer and neurodegenerative diseases. Its capability to regulate cell cycle and apoptosis connects it to cancer biology where altered expression or activity might contribute to unchecked cell proliferation. Variations in KAT2B/PCAF activity also link to neurodegenerative disorders like Huntington’s disease. The protein interacts with other factors like p53 and SMAD transcription factors in contexts relevant to these diseases suggesting its roles in pathways critical for disease development or progression.