Abcam, ab300307, Human Factor IX/PTC ELISA Kit

Size: 96Tests / 1 x 384Tests / 10 x 96Tests
Human Factor IX/PTC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor IX/PTC with a sensitivity of 15.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 15.884 pg/mL,
Range:97.656 - 6250 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Factor IX/PTC ELISA Kit ab300307 is a rapid single-wash 90-min ELISA kit to measure Human Factor IX/PTC in serum and plasma. This SimpleStep ELISA® sensitivity is 15.884 pg/mL.
How the assay works
Human Factor IX/PTC SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human Factor IX/PTC. The standard protein in this kit is full length human Factor IX/PTC. Native signal was detected in serum, plasma (heparin), plasma (EDTA), and plasma (citrate). Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.
Human Factor IX/PTC ELISA Kit ab300307 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Save your precious samples
Our 384-well Human Factor IX/PTC ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Factor IX (F9) Antibody Pair - BSA and Azide free
ab253721
- Anti-Factor IX/PTC antibody [EPR22846-57] - BSA and Azide free (Capture)
ab281281
- Anti-Factor IX/PTC antibody [EPR22846-233] - BSA and Azide free (Detector)
ab281131
Related and recommended products
Human Factor IX/PTC ELISA Kit ab300307 is commonly used to study cardiovascular diseases and clotting disorders.
Other ELISA kits to measure inflammation and coagulation include:
- Human Factor X ELISA Kit
ab108832
- Human Lipocalin-2 ELISA Kit
ab215541

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C