Abcam, ab300318, Human Factor B ELISA Kit

Size: 96Tests
Human Factor B ELISA Kit is a sandwich ELISA designed to quantify Human Factor B with a sensitivity of 18.18 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 53.125 - 3400 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 18.18 pg/mL,
Range:53.125 - 3400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Factor B ELISA Kit ab300318 is a rapid single-wash 90-min Sandwich ELISA to measure Human Factor B in cell culture supernatant, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 18.18 pg/mL.
How the assay works
Human Factor B SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Factor B ELISA Kit ab300318 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture)
ab302723
- Anti-Factor B antibody [EPR24287-95] - BSA and Azide free (Detector)
ab302723

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
Biological function summary
Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.
Pathways
Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.
Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.