Abcam, ab300490, Anti-NSD3 antibody [EPR25189-8] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NSD3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25189-8,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:IP, WB, ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NSD3 also known as WHSC1L1 is a protein involved in gene expression regulation through its role as a histone methyltransferase. It has a molecular mass of approximately 170 kDa. This protein specifically modifies lysine residues on histone tails which influences chromatin structure and gene transcription. NSD3 is broadly expressed in various tissues but higher levels appear in actively dividing cells such as those found in the brain and reproductive organs.
Biological function summary
NSD3 plays an important role in the regulation of chromatin state and is part of the larger SETD2 family of methyltransferases. It forms part of complexes that influence transcriptional activation and repression by altering chromatin accessibility. These complexes allow NSD3 to exert control over genes involved in cell proliferation and differentiation impacting cellular processes critical to development and tissue maintenance.
Pathways
NSD3 integrates into chromatin remodeling and gene expression pathways. It interacts with other methyltransferases and proteins like NSD1 and NSD2 to regulate transcriptional programs essential for cellular growth. It is part of pathways that modulate key processes such as the cell cycle and DNA damage response indicating its role in maintaining genomic stability and regulating cell fate decisions.
NSD3 has implications in cancer development and progression. It is particularly linked to certain types of breast cancer where overexpression leads to uncontrolled cell growth. In these cases NSD3 influences oncogenic pathways and interacts with proteins such as BRD4 to drive tumorigenesis. Its dysregulation might also contribute to neurodevelopmental disorders by affecting proteins involved in neural development and connectivity.