Abcam, ab300516, Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TRIM9 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25103-71,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIM9 also known as Tripartite Motif Containing 9 is an E3 ubiquitin ligase with a mass of approximately 68 kDa. This protein adds ubiquitin to substrate proteins tagging them for proteasomal degradation or altering their activity. TRIM9 contains a RING domain B-box type zinc finger and coiled-coil region typical of the TRIM family proteins. It is widely expressed in the nervous system with high levels found in the brain highlighting its significance in neural processes.
Biological function summary
The TRIM9 protein regulates axon pathfinding and synapse formation influencing neural network development. TRIM9 forms complexes with other proteins to modulate cytoskeletal dynamics affecting neuron shape and connectivity. By interacting with proteins like DCC (Deleted in Colorectal Carcinoma) TRIM9 plays a role in guiding axons during neural circuit formation. This interaction is essential for creating proper neuronal connections and ensuring functional communication within the brain.
Pathways
TRIM9 emerges as an important regulator in the Netrin-1 signaling pathway critical for axonal guidance. This pathway involves interaction with DCC a receptor for Netrin-1 influencing axonal steering decisions. Additionally TRIM9 participates in the ubiquitination pathway affecting protein stability and cellular localization that modulates various signaling cascades within neurons. By manipulating these pathways TRIM9 assists in neural development and plasticity.
Alterations in TRIM9 expression or function correlate with neurological conditions such as schizophrenia and autism spectrum disorders. Dysfunctional TRIM9-mediated pathways might disrupt neural circuit development contributing to the cognitive and social impairments associated with these disorders. Other proteins like DCC also share connections with these disorders indicating a network of interactions where TRIM9 influences disease pathology.